Young-Sam Lee joined the Department of Biology in 2010. He previously worked as a postdoctoral fellow at Harvard University. His lab is interested in protein-metabolite interactions and how they contribute to cellular adaptation.
Our lab is interested in protein-metabolite interactions and how they contributes to cellular adaptation. Understanding how cells sense and adapt to new environment is an important question relevant to human health conditions. In particular, we are interested in the contribution of cellular metabolism to the adaptation process.
When cells are exposed to a new environment, their intracellular metabolic profile can change rapidly and drastically. This change can lead to new protein-metabolite interactions, which then serve as a mechanism for cells to sense the new environment. In pathological conditions, these protein-metabolite interactions can be altered and contribute to the disease progression.
In our lab, using liquid chromatography and mass spectrometry, we identify cellular metabolites that regulate clinically important proteins. We also use biochemical and cell biological methods to determine the role of these metabolite-protein interactions in normal and pathological metabolic adaptations.
Yan M, Chakravarthy S, Tokuda JM, Pollack L, Bowman GD*, & Lee YS* (2016) Succinyl-5-aminoimidazole-4-carboxamide-1-ribose 5′-Phosphate (SAICAR) activates pyruvate kinase isoform M2 (PKM2) in its dimeric form. Biochemistry 55(33) 4731-4736 [PMID: 27481063]
Kim J, Xiao H, Koh J, Wang Y, Bonanno JB, Thomas K, Babbitt PC, Brown S, Lee YS, & Almo SC. (2015) Determinants of the ComB carboxymethyl transferase utilized for selective tRNA wobble modification. Nucleic Acids Research 43(9), 4602-4613 [PMCID: PMC4482062]
Keller KE, Doctor ZM, Dwyer ZW, & Lee YS* (2014) SAICAR induces protein kinase activity of PKM2 that is necessary for sustained proliferative signaling of cancer cells. Molecular Cell 53(5), 700-709 [PMID: 24606918]
Kim J. Xiao H, Bonanno JB, Kalyanaraman C, Brown S, Tang X, Patskovsky Y, Babbitt PC, Jacobson MP,Lee YS, & Almo SC (2013) Structure-guided discovery of the metabolite carboxy-SAM that modulates tRNA function. Nature 498, 123-126 [PMID: 23676670]
Keller KE, Tan IS, & Lee YS* (2012) SAICAR stimulates pyruvate kinase isoform M2 and promotes cancer cell survival in glucose-limited conditions. Science 338, 1069-1072 [PMC3527123]
Lee YS, Huang K, Quiocho FA, O'Shea EK. (2008) Molecular basis of cyclin-CDK-CKI regulation by reversible binding of an inositol pyrophosphate. Nat. Chem. Biol. 4, 25 - 32. [Pubmed]
Lee YS, Mulugu S, York JD, O'Shea EK (2007) Regulation of a cyclin-CDK-CDK inhibitor complex by inositol pyrophosphates. Science 316, 109 -112. [Pubmed]
2016 Catalyst award, Johns Hopkins University
2013 Kimmel Scholar, Sidney Kimmel Foundation for Cancer Research
2007 Helen Hay Whitney Foundation Post-doctoral Fellowship
2002 Burroughs Wellcome Funds Interfaces at Science Program Pre-doctoral Fellowship