Ian Dobbie

Ian Dobbie

Research Professor and Director of the Integrated Imaging Center

PhD, Randall Institute, King’s College London

ian.dobbie@jhu.edu

Ian Dobbie is a Research Professor in the Department of Biology and Director of the Integrated Imaging Center at the Homewood Campus. His research focuses on building novel super-resolution fluorescence microscopes for cutting edge bioimaging studies.

Publications

  1. A. OkoloI. KounatidisJ. GroenK.L. NahasS. BalintT.M. FishM.A. KoronfelA.L. CortajarenaI.M. DobbieE. PereiroM. Harkiolaki (2021). Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures. Nature Protocols volume 16, 2851–2885
  2. Wang, E.S. Allgeyer, G. Sirinakis, Y. Zhang, K. Hu, M.D. Lessard, Y. Li, R. Diekmann, M.A. Phillips, I.M. Dobbie*, J. Ries*, M.J. Booth*, J. Bewersdorf* (2020). Implementation of a 4Pi-SMS Super-Resolution Microscope. Nature Protocols in press (* joint last authors)
  3. A. Hussain, T. Kubo, N. Hall, D. Gala, K. Hampson, R. Parton, M.A. Phillips, M. Wincott, K. Fujita, I. Davis, I.M Dobbie, Martin J. Booth (2020) Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope. Journal of Microscopy in press
  4. Kounatidis,  M.L. Stanifer,  M.A. Phillips,  P. Paul-Gilloteaux,  X. Helligenstein,  H. Wang,   C.A. Okolo,  T.M. Fish,  M.C. Spink,  D.I. Stuart,  I. Davis,  S. Boulant,  J.M. Grimes,  I.M. Dobbie,  M. Harkiolaki (2020). Correlative cryo-structured illumination fluorescence microscopy and soft X-ray tomography elucidates reovirus intracellular release pathway. Cell 182 (2), 515-530
  5. A. Phillips, M. Harkiolaki, D.M Susano Pinto, R.M. Parton, A. Palanca, M. Garcia-Moreno, I Kounatidis, JW Sedat, D.I. Stuart, A. Castello, M.J. Booth, I. Davis, and I.M. Dobbie (2020). CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging. Optica 7 (7) 802-812
  6. Hall, J. Titlow, M.J. Booth, I.M. Dobbie (2020). Microscope-AOtools: A generalised adaptive optics implementation. Optics Express 28 (20), 28987-29003
  7. J Hall, D.M.S Pinto, I.M. Dobbie (2019). BeamDelta: simple alignment tool for optical systems. Wellcome Open Research 4, 194
  8. M Žurauskas, M. Dobbie, RM Parton, MA Phillips, A Göhler, I Davis, M.J. Booth (2019). IsoSense: frequency enhanced sensorless adaptive optics through structured illumination. Optica 6 (3), 370-379
  9. A. Phillips, D.M. Susano Pinto, I.M. Dobbie (2018). SPEKcheck—fluorescence microscopy spectral visualisation and optimisation: a web application, javascript library, and data resource. Wellcome Open Research 3
  10. Gartenmann, A. Wainman, M. Quarashi, R. Kaufmann, S. Schubert, J.W. Raff, I.M Dobbie (2017) A Combined 3D-SIM/SMLM approach allows centriole proteins to be localized with a precision of ~4-5nm. Current Biology 27 (19), R1054-R1055.
  11. Demmerle, C. Innocent, A.J. North, G. Ball, M. Müller, E. Miron, A. Matsuda, I.M. Dobbie, Y. Markaki, L. Schermelleh (2017). Strategic and practical guidelines for successful structured illumination microscopy. Nature Protocols 12 (5), 988
  12. WegelA. GöhlerB. C. LagerholmA. WainmanS. UphoffR. Kaufmann, I. M. Dobbie (2016). Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison. Scientific Reports 6, Article number: 27290
  13. Demmerle, E. Wegel, L. Schermelleh, I.M. Dobbie (2015) Assessing Resolution in Super-resolution imaging. Methods 88, 3-10.
  14. Ball, J. Demmerle, R. Kaufmann, I. Davis, I. M. Dobbie, L. Schermelleh (2015). SIMcheck: a Toolbox for Successful Super-resolution SIM Imaging. Sci Rep 5, 15915
  15. PT Conduit, JH Richens, A Wainman, J Holder, CC Vicente, MB Pratt, C.I. Dix, Z.A. Novak, M. Dobbie, L. Schermelleh, J.W. Raff (2014). A molecular mechanism of mitotic centrosome assembly in Drosophila. eLife 3, e03399
  16. R Kaufmann, P Schellenberger, E Seiradake, I Dobbie, EY Jones, I Davis . C. Hagen, K. Grunewald (2014). Super-Resolution Microscopy using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions. Nano letters 14(7), 4171-5
  17. C.N. Brown, I.M. Dobbie, J.M. Alakoskela, I. Davis, D.M. Davis (2012). Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood, 120(18), 3729–3740.
  18. C.N. Brown, S. Oddos, I.M. Dobbie, J.M. Alakoskela, R.M. Parton, P. Eissmann, M. A.A. Neil, C. Dunsby, P.M.W. French, I. Davis, D. M. Davis. (2012). Remodelling of Cortical Actin Where Lytic Granules Dock at Natural Killer Cell Immune Synapses Revealed by Super-Resolution Microscopy. PLoS biology 9 (9), e1001152
  19. J. Delalez, G.H. Wadhams, G. Rosser, Q. Xue, M.T. Brown, I.M. Dobbie,  R.M. Berry, M.C. Leake, J.P. Armitage (2010). Signal-dependent turnover of the bacterial flagellar switch protein FliM. Proceedings of the National Academy of Sciences of the United States of America, 107(25), 11347–11351.
  20. M. Dobbie, A. Robson, N. Delalez, M.C. Leake (2009). Visualizing single molecular complexes in vivo using advanced fluorescence microscopy. Journal of Visualized Experiments : JoVE, (31), 1508.
  21. T. Weil, R.M. Parton, B. Herpers, J. Soetaert, T. Veenendaal, D. Xanthakis, I.M. Dobbie, J.M. Halstead, R. Hayashi,C. Rabouille, I. Davis (2012). Drosophila patterning is established by differential association of mRNAs with P bodies. Nature Cell Biology, 14(12), 1305–1315. doi:doi:10.1038/ncb2627
  22. T Weil, D. Xanthakis, R. Parton, I. Dobbie, C. Rabouille, E.R. Gavis, I. Davis (2010). Distinguishing direct from indirect roles for bicoid mRNA localization factors. Development (Cambridge, England), 137(1), 169–176.
  23. Braet*, H Stephan*, I.M. Dobbie*, D.M. Togashi, A.G. Ryder, Z. Földes-Papp, N.F. Lowndes, H.P. Nasheuer. Mobility and Distribution of Replication Protein A in Living Cells Using Fluorescent Correlation Spectroscopy. Experimental & Molecular Pathology. Vol. 82(2), pp 156-6212 (2007). *Joint first authors.
  24. W. Toh, A.M. O'Shaughnessy, S. Jimeno, I.M. Dobbie, M. Grenon, S. Maffini, A. O'Rorke N.F. Lowndes. Histone H2A phosphorylation and H3 methylation are required for a novel Rad9 DSB repair function following checkpoint activation. DNA Repair Vol. 5(6), pp 693-703 (2006)
  25. Zicha, I. Dobbie, M.R. Holt, J. Monypenny, D.Y. Soong, C. Gray, G.A. Dunn. Rapid actin transport during cell protrusion. Science Vol 300, pp 142-145 (2003).
  26. Reconditi, N. Koubassova, M. Linari, I. Dobbie, T. Narayanan, O. Diat, G. Piazzesi, V. Lombardi, M. Irving. The conformation of myosin head domains in rigor muscle determined by X-ray interference. Biophysical Journal, Vol. 85, pp 1098-1110 (2003).
  27. A. Dunn, I. Dobbie, J. Monypenny, M. R. Holt, D. Zicha. Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells. Journal of Microscopy Vol 205(1), pp 109-113 (2002)
  28. Linari, G. Piazzesi, I. Dobbie, N. Koubassova, M. Reconditi, T. Narayanan, O. Diat, M. Irving & V. Lombardi. Interference Fine Structure and Sarcomere Length Dependence of the Axial X-Ray Pattern from Active Single Muscle Fibers. PNAS, Vol 97(13), pp 7226-7231 (2000).
  29. Irving, G. Piazzesi, L Lucii, Y. B. Sun, J. J. Harford, I. Dobbie, M. A. Farenczi, M. Reconditi & V. Lombardi. Conformation of the Myosin Motor During Force Generation in Skeletal Muscle. Nature Structural Biology, Vol 7(6), pp 482-485 (2000).
  30. Piazzesi, M. Reconditi, I. Dobbie, M. Linari, P. Boesecke, O. Diat, M. Irving and V. Lombardi. Changes in Conformation of Myosin Heads During the Development of Isometric Contraction and Rapid Shortening in Single Frog Muscle Fibres. Journal of Physiology, Vol 514, pp 305-312 (1999).
  31. Reconditi, I. Dobbie, M. Irving, O. Diat, P. Boesecke, M. Linari, G. Piazzesi and V. Lombardi. Myosin Head Movements During Isometric Contraction Studied by X-ray Diffraction of Single Frog Muscle Fibres. Advances in Experimental Medicine and Biology Vol 453, pp 265-270 (1998).
  32. Dobbie, M. Linari, G. Piazzesi, M. Reconditi, N. Koubassova, M. A. Ferenczi, V. Lombardi and M. Irving. Elastic Bending and Active Tilting of Myosin Heads During Muscle Contraction. Nature, Vol 396, pp 383-387 (1998).
  33. Linari, I. Dobbie, M. Reconditi, N. Koubassova, M. Irving, G. Piazzesi and V. Lombardi. The stiffness of Skeletal Muscle in Isometric Contraction and Rigor: The Fraction of Myosin Heads Bound to Actin. Biophysical Journal, Vol. 74, pp 2459-2473 (1998).
  34. Lombardi, G. Piazzesi, M. A. Ferenczi, H. Thirlwell, I. Dobbie and M. Irving. Elastic Distortion of Myosin Heads and Repriming of the Working Stroke in Muscle. Nature, Vol. 374, pp. 552-555 (1995).
  35. Piazzesi, V. Lombardi, M. A. Ferenczi, H. Thirlwell, I. Dobbie and M. Irving. Changes in the X-Ray Diffraction Pattern from Single, Intact Muscle Fibres Produced by Rapid Shortening and Stretch. Biophysical Journal, Vol. 68, pp 92s-98s (1995).

Book Chapters

  1. M. Dobbie, E. King, R.M. Parton, P.M. Carlton, J.W. Sedat, J.R. Swedlow, I. Davis. OMX: a new platform for multimodal, multichannel wide-field imaging. Cold Spring Harbor protocols 8 pp 121, Cold Spring Harbor Laboratory Press (2011)
  2. M. Parton, A.M. Vallés, I.M. Dobbie, I. Davis. Live cell imaging in Drosophila melanogaster. Cold Spring Harbor Protocols, 4, pp 75, Cold Spring Harbor Laboratory Press (2010)
  3. M. Dobbie, N.F. Lowndes, K.F. Sullivan. Autofluorescent proteins. Methods in cell biology 85, pp 1-22, Elsevier (2008)
  4. R. Holt, D.Y.H. Soong, J. Monypenny, I.M. Dobbie, D. Zicha, G.A. Dunn. Using bioprobes to follow protein dynamics in living cells. Cell Motility: From Molecules to Organisms, pp 117-134, John Wiley & Sons, Ltd (2004)
  5. Dobbie & D. Zicha. Microscopy of Living Cells. Basic Cell Culture: A Practical Approach 2nd Edition. OUP (2002).