Associate Research Professor
Jocelyne DiRuggiero is an associate research professor in the Department of Biology and holds an appointment in the Department of Earth and Planetary Sciences. She received her PhD from the University Claude Bernard in Lyon, France. Her research focuses on the mechanisms of stress response in extremophiles and on the microbial ecology of extreme environments.
Microorganisms have inhabited the Earth for 3.4 billion years of its history. They are essential for the evolution of its minerals, its major geochemical cycles, and its atmosphere, yet the extent of their diversity, their metabolic capabilities, and their ecosystem-level interactions remains vastly unexplored.
Understanding the link between the geochemistry we observe and the large-scale impact of microbial activity is critical if we want to learn more about our own biosphere, and how to better conserve and protect the environment. We study microorganisms that live in extreme environments, such as hypersaline environments and extremely dry deserts, because under those extreme conditions - where the impact on cellular components is intense - microorganisms have evolved robust adaptive mechanisms.
Our work focuses on processes that allow microorganisms to adapt to their environments and to changing environmental conditions. We are particularly interested in mechanisms of resistance to oxidative stress, desiccation, and radiation. We use a combination of genomic and genetic methods to identify proteins involved in the repair of DNA damage in the Archaea and to elucidate the regulatory networks underlying the stress response to cellular damage.
At the ecosystem level, we study the genetic and functional diversity of microbial communities from extreme environments to identify the mechanisms that sustain biodiversity and ecosystem functioning. Our field sites include the Atacama Desert, Chile, and the Dry Valleys of Antarctica, both extremely dry areas and Earth analogs for Martian environments.
Our research is directly related to Astrobiology. Visit the Institute for Planets and Life web site at the Space Telescope Science Institute and Johns Hopkins University
Atacama Desert, Chile
Crits-Christoph, A., C. K. Robinson, B. Ma, J. Ravel, J. Wierzchos, C. Ascaso, O. Artieda, V. Souza-Egipsy, C. Casero, and J. DiRuggiero. 2016. Phylogenetic and functional substrate specificity for endolithic microbial communities in hyper-arid environments. Frontiers Microbiol. http://dx.doi.org/10.3389/fmicb.2016.00301
Crits-Christoph, A. D. R. Gelsinger, B. Ma, J. Wierzchos, J. Ravel, A. F. Davila, M. C. Casero, and J. DiRuggiero. 2016. Functional interactions of archaea, bacteria and viruses in a hypersaline endolithic community. Env. Microbiol. DOI: 10.1111/1462-2920.13259
Goordial, J, A. Davila, D. Lacelle, W. Pollard, M.M Marinova, C.W. Greer, J. DiRuggiero, C.P. McKay and L.G. Whyte. 2015. Nearing the cold-arid limits of microbial life in permafrost of an upper dry valley, Antarctica. ISMEJ in press.
Davila, A.F., I. Hawes, J. Garcia, D.R. Gelsinger. J. DiRuggiero, C. Ascaso, A. Osano, and J. Wierzchos. 2015. In situ metabolism in halite endolithic microbial communities of the hyperarid Atacama Desert. Frontiers Microbiol. 6:1035. http://dx.doi.org/10.3389/fmicb.2015.01035
Wierzchos, J., J. DiRuggiero, P. Vítek, O. Artieda, V. Souza-Egipsy, P. Skaloud, M. Tisza, A. F. Davila, C. Vílchez, I. Garbayo, and C. Ascaso. 2015. Adaptation strategies of endolithic chlorophototrophs to survive the hyperarid and extreme solar radiation environment of the Atacama Desert. Frontiers Microbiol. 6: 934. http://dx.doi.org/10.3389/fmicb.2015.00934
Robinson, C. K., J. Wierzchos, C. Black, A. Crits-Christoph, B. Ma, J. Ravel, C. Ascaso, O. Artieda, S. Valea, M. Roldan, B. Gomez-Silva, and J. DiRuggiero. 2015. Microbial diversity and the presence of algae in halite endolithic communities are correlated to atmospheric moisture in the hyper-arid zone of the Atacama Desert. Environ Microbiol doi: 17: 299-315
Crits-Christoph, A., C. K. Robinson, T. Barnum, J. Ravel, F. Fricke, A. Davila, B. Jedynak, C.P. McKay, and J. DiRuggiero. 2013. Colonization patterns of soil microbial communities in the Atacama Desert. Microbiome 1:28.
DiRuggiero, J., J. Wierzchos, C. K. Robinson, T. Souterre, R. Ravel, O. Artieda, V. Souza- Egipsy, and C. Ascaso. 2013. Microbial colonization of chasmoendolithic habitats in the hyper-arid zone of the Atacama Desert. Biogeosciences 10:2439-2450
Webb, K., J. Wu, C.K. Robinson, N. Tomiya, Y. Lee, and J. DiRuggiero. 2013. Effects of intracellular Mn on the radiation resistance of the halophilic archaeon Halobacterium salinarum. Extremophiles. 17: 485-497
Webb, K.M., and J. DiRuggiero. 2012. Role of Mn2+ and compatible solutes in the radiation resistance of thermophilic bacteria and archaea. Archaea. 2012:Article ID 845756. doi:10.1155/2012/845756
Robinson, C.K., K.M. Webb, A. Kaur, P. Jaruga, M. Dizdaroglu, N. Baliga, A. Place, and J. DiRuggiero. 2011. A major role for non-enzymatic antioxidant processes in the radioresistance of Halobacterium salinarum. J. Bacteriol. 193:1653-1662
Kaur, A., P.T. Van, C.R. Busch, C. Robinson, M. Pan, W.L. Pang, D. Reiss, J. DiRuggiero, and N.S. Baliga. 2010. Coordination of frontline defense mechanisms under severe oxidative stress. Mol. Syst. Biol. 393 doi:10.1038/msb.2010.50
Kish, A., G. Kirkali, C. Robinson, R. Rosenblatt, P. Jaruga, M. Dizdaroglu, and J. DiRuggiero. 2009. Salt shield: intracellular salts provide protection against ionizing radiation in the halophilic archaeon, Halobacterium salinarum NRC-1 Environ. Microbiol. 11:1066-1078.